Screening and identification of linear B-cell epitopes and entry-blocking peptide of severe acute respiratory syndrome (SARS)-associated coronavirus using synthetic overlapping peptide library.
Identifieur interne : 006703 ( Main/Exploration ); précédent : 006702; suivant : 006704Screening and identification of linear B-cell epitopes and entry-blocking peptide of severe acute respiratory syndrome (SARS)-associated coronavirus using synthetic overlapping peptide library.
Auteurs : Hongbo Hu [République populaire de Chine] ; Li Li ; Richard Y. Kao ; Binbin Kou ; Zhanguo Wang ; Liang Zhang ; Huiyuan Zhang ; Zhiyong Hao ; Wayne H. Tsui ; Anping Ni ; Lianxian Cui ; Baoxing Fan ; Feng Guo ; Shuan Rao ; Chengyu Jiang ; Qian Li ; Manji Sun ; Wei He ; Gang LiuSource :
- Journal of combinatorial chemistry [ 1520-4766 ]
Descripteurs français
- KwdFr :
- Antigènes viraux (), Antigènes viraux (génétique), Antigènes viraux (immunologie), Banque de peptides, Données de séquences moléculaires, Déterminants antigéniques des lymphocytes B (), Déterminants antigéniques des lymphocytes B (génétique), Déterminants antigéniques des lymphocytes B (immunologie), Syndrome respiratoire aigu sévère (immunologie), Séquence d'acides aminés, Techniques de chimie combinatoire, Virus du SRAS (), Virus du SRAS (immunologie).
- MESH :
- génétique : Antigènes viraux, Déterminants antigéniques des lymphocytes B.
- immunologie : Antigènes viraux, Déterminants antigéniques des lymphocytes B, Syndrome respiratoire aigu sévère, Virus du SRAS.
- Antigènes viraux, Banque de peptides, Données de séquences moléculaires, Déterminants antigéniques des lymphocytes B, Séquence d'acides aminés, Techniques de chimie combinatoire, Virus du SRAS.
English descriptors
- KwdEn :
- Amino Acid Sequence, Antigens, Viral (chemistry), Antigens, Viral (genetics), Antigens, Viral (immunology), Combinatorial Chemistry Techniques, Epitopes, B-Lymphocyte (chemistry), Epitopes, B-Lymphocyte (genetics), Epitopes, B-Lymphocyte (immunology), Molecular Sequence Data, Peptide Library, SARS Virus (chemistry), SARS Virus (immunology), Severe Acute Respiratory Syndrome (immunology).
- MESH :
- chemical , chemistry : Antigens, Viral, Epitopes, B-Lymphocyte.
- chemical , genetics : Antigens, Viral, Epitopes, B-Lymphocyte.
- chemical , immunology : Antigens, Viral, Epitopes, B-Lymphocyte.
- chemistry : SARS Virus.
- immunology : SARS Virus, Severe Acute Respiratory Syndrome.
- Amino Acid Sequence, Combinatorial Chemistry Techniques, Molecular Sequence Data, Peptide Library.
Abstract
A 10-mer overlapping peptide library has been synthesized for screening and identification of linear B-cell epitopes of severe acute respiratory syndrome associated coronavirus (SARS-CoV), which spanned the major structural proteins of SARS-CoV. One hundred and eleven candidate peptides were positive according to the result of PEPscan, which were assembled into 22 longer peptides. Five of these peptides showed high cross-immunoreactivities (approximately 66.7 to 90.5%) to SARS convalescent patients' sera from the severest epidemic regions of the China mainland. Most interestingly, S(471-503), a peptide located at the receptor binding domain (RBD) of SARS-CoV, could specifically block the binding between the RBD and angiotensin-converting enzyme 2, resulting in the inhibition of SARS-CoV entrance into host cells in vitro. The study demonstrated that S(471-503) peptide was a potential immunoantigen for the development of peptide-based vaccine or a candidate for further drug evaluation against the SARS-CoV virus-cell fusion.
DOI: 10.1021/cc0500607
PubMed: 16153058
Affiliations:
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Le document en format XML
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China.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, 1 Xian Nong Tan Street, Beijing 100050</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Li, Li" sort="Li, Li" uniqKey="Li L" first="Li" last="Li">Li Li</name></author><author><name sortKey="Kao, Richard Y" sort="Kao, Richard Y" uniqKey="Kao R" first="Richard Y" last="Kao">Richard Y. 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Tsui</name></author><author><name sortKey="Ni, Anping" sort="Ni, Anping" uniqKey="Ni A" first="Anping" last="Ni">Anping Ni</name></author><author><name sortKey="Cui, Lianxian" sort="Cui, Lianxian" uniqKey="Cui L" first="Lianxian" last="Cui">Lianxian Cui</name></author><author><name sortKey="Fan, Baoxing" sort="Fan, Baoxing" uniqKey="Fan B" first="Baoxing" last="Fan">Baoxing Fan</name></author><author><name sortKey="Guo, Feng" sort="Guo, Feng" uniqKey="Guo F" first="Feng" last="Guo">Feng Guo</name></author><author><name sortKey="Rao, Shuan" sort="Rao, Shuan" uniqKey="Rao S" first="Shuan" last="Rao">Shuan Rao</name></author><author><name sortKey="Jiang, Chengyu" sort="Jiang, Chengyu" uniqKey="Jiang C" first="Chengyu" last="Jiang">Chengyu Jiang</name></author><author><name sortKey="Li, Qian" sort="Li, Qian" uniqKey="Li Q" first="Qian" last="Li">Qian Li</name></author><author><name sortKey="Sun, Manji" sort="Sun, Manji" uniqKey="Sun M" first="Manji" last="Sun">Manji Sun</name></author><author><name sortKey="He, Wei" sort="He, Wei" uniqKey="He W" first="Wei" last="He">Wei He</name></author><author><name sortKey="Liu, Gang" sort="Liu, Gang" uniqKey="Liu G" first="Gang" last="Liu">Gang Liu</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="????"><PubDate><MedlineDate>2005 Sep-Oct</MedlineDate></PubDate></date><idno type="RBID">pubmed:16153058</idno><idno type="pmid">16153058</idno><idno type="doi">10.1021/cc0500607</idno><idno type="wicri:Area/PubMed/Corpus">002550</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002550</idno><idno type="wicri:Area/PubMed/Curation">002550</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002550</idno><idno type="wicri:Area/PubMed/Checkpoint">003519</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">003519</idno><idno type="wicri:Area/Ncbi/Merge">001152</idno><idno type="wicri:Area/Ncbi/Curation">001152</idno><idno type="wicri:Area/Ncbi/Checkpoint">001152</idno><idno type="wicri:doubleKey">1520-4766::Hu H:screening:and:identification</idno><idno type="wicri:Area/Main/Merge">006C21</idno><idno type="wicri:Area/Main/Curation">006703</idno><idno type="wicri:Area/Main/Exploration">006703</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Screening and identification of linear B-cell epitopes and entry-blocking peptide of severe acute respiratory syndrome (SARS)-associated coronavirus using synthetic overlapping peptide library.</title><author><name sortKey="Hu, Hongbo" sort="Hu, Hongbo" uniqKey="Hu H" first="Hongbo" last="Hu">Hongbo Hu</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, 1 Xian Nong Tan Street, Beijing 100050, P. R. China.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, 1 Xian Nong Tan Street, Beijing 100050</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Li, Li" sort="Li, Li" uniqKey="Li L" first="Li" last="Li">Li Li</name></author><author><name sortKey="Kao, Richard Y" sort="Kao, Richard Y" uniqKey="Kao R" first="Richard Y" last="Kao">Richard Y. Kao</name></author><author><name sortKey="Kou, Binbin" sort="Kou, Binbin" uniqKey="Kou B" first="Binbin" last="Kou">Binbin Kou</name></author><author><name sortKey="Wang, Zhanguo" sort="Wang, Zhanguo" uniqKey="Wang Z" first="Zhanguo" last="Wang">Zhanguo Wang</name></author><author><name sortKey="Zhang, Liang" sort="Zhang, Liang" uniqKey="Zhang L" first="Liang" last="Zhang">Liang Zhang</name></author><author><name sortKey="Zhang, Huiyuan" sort="Zhang, Huiyuan" uniqKey="Zhang H" first="Huiyuan" last="Zhang">Huiyuan Zhang</name></author><author><name sortKey="Hao, Zhiyong" sort="Hao, Zhiyong" uniqKey="Hao Z" first="Zhiyong" last="Hao">Zhiyong Hao</name></author><author><name sortKey="Tsui, Wayne H" sort="Tsui, Wayne H" uniqKey="Tsui W" first="Wayne H" last="Tsui">Wayne H. Tsui</name></author><author><name sortKey="Ni, Anping" sort="Ni, Anping" uniqKey="Ni A" first="Anping" last="Ni">Anping Ni</name></author><author><name sortKey="Cui, Lianxian" sort="Cui, Lianxian" uniqKey="Cui L" first="Lianxian" last="Cui">Lianxian Cui</name></author><author><name sortKey="Fan, Baoxing" sort="Fan, Baoxing" uniqKey="Fan B" first="Baoxing" last="Fan">Baoxing Fan</name></author><author><name sortKey="Guo, Feng" sort="Guo, Feng" uniqKey="Guo F" first="Feng" last="Guo">Feng Guo</name></author><author><name sortKey="Rao, Shuan" sort="Rao, Shuan" uniqKey="Rao S" first="Shuan" last="Rao">Shuan Rao</name></author><author><name sortKey="Jiang, Chengyu" sort="Jiang, Chengyu" uniqKey="Jiang C" first="Chengyu" last="Jiang">Chengyu Jiang</name></author><author><name sortKey="Li, Qian" sort="Li, Qian" uniqKey="Li Q" first="Qian" last="Li">Qian Li</name></author><author><name sortKey="Sun, Manji" sort="Sun, Manji" uniqKey="Sun M" first="Manji" last="Sun">Manji Sun</name></author><author><name sortKey="He, Wei" sort="He, Wei" uniqKey="He W" first="Wei" last="He">Wei He</name></author><author><name sortKey="Liu, Gang" sort="Liu, Gang" uniqKey="Liu G" first="Gang" last="Liu">Gang Liu</name></author></analytic><series><title level="j">Journal of combinatorial chemistry</title><idno type="ISSN">1520-4766</idno></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Antigens, Viral (chemistry)</term><term>Antigens, Viral (genetics)</term><term>Antigens, Viral (immunology)</term><term>Combinatorial Chemistry Techniques</term><term>Epitopes, B-Lymphocyte (chemistry)</term><term>Epitopes, B-Lymphocyte (genetics)</term><term>Epitopes, B-Lymphocyte (immunology)</term><term>Molecular Sequence Data</term><term>Peptide Library</term><term>SARS Virus (chemistry)</term><term>SARS Virus (immunology)</term><term>Severe Acute Respiratory Syndrome (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Antigènes viraux ()</term><term>Antigènes viraux (génétique)</term><term>Antigènes viraux (immunologie)</term><term>Banque de peptides</term><term>Données de séquences moléculaires</term><term>Déterminants antigéniques des lymphocytes B ()</term><term>Déterminants antigéniques des lymphocytes B (génétique)</term><term>Déterminants antigéniques des lymphocytes B (immunologie)</term><term>Syndrome respiratoire aigu sévère (immunologie)</term><term>Séquence d'acides aminés</term><term>Techniques de chimie combinatoire</term><term>Virus du SRAS ()</term><term>Virus du SRAS (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Antigens, Viral</term><term>Epitopes, B-Lymphocyte</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Antigens, Viral</term><term>Epitopes, B-Lymphocyte</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antigens, Viral</term><term>Epitopes, B-Lymphocyte</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Antigènes viraux</term><term>Déterminants antigéniques des lymphocytes B</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Antigènes viraux</term><term>Déterminants antigéniques des lymphocytes B</term><term>Syndrome respiratoire aigu sévère</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Combinatorial Chemistry Techniques</term><term>Molecular Sequence Data</term><term>Peptide Library</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Antigènes viraux</term><term>Banque de peptides</term><term>Données de séquences moléculaires</term><term>Déterminants antigéniques des lymphocytes B</term><term>Séquence d'acides aminés</term><term>Techniques de chimie combinatoire</term><term>Virus du SRAS</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A 10-mer overlapping peptide library has been synthesized for screening and identification of linear B-cell epitopes of severe acute respiratory syndrome associated coronavirus (SARS-CoV), which spanned the major structural proteins of SARS-CoV. One hundred and eleven candidate peptides were positive according to the result of PEPscan, which were assembled into 22 longer peptides. Five of these peptides showed high cross-immunoreactivities (approximately 66.7 to 90.5%) to SARS convalescent patients' sera from the severest epidemic regions of the China mainland. Most interestingly, S(471-503), a peptide located at the receptor binding domain (RBD) of SARS-CoV, could specifically block the binding between the RBD and angiotensin-converting enzyme 2, resulting in the inhibition of SARS-CoV entrance into host cells in vitro. The study demonstrated that S(471-503) peptide was a potential immunoantigen for the development of peptide-based vaccine or a candidate for further drug evaluation against the SARS-CoV virus-cell fusion.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><noCountry><name sortKey="Cui, Lianxian" sort="Cui, Lianxian" uniqKey="Cui L" first="Lianxian" last="Cui">Lianxian Cui</name><name sortKey="Fan, Baoxing" sort="Fan, Baoxing" uniqKey="Fan B" first="Baoxing" last="Fan">Baoxing Fan</name><name sortKey="Guo, Feng" sort="Guo, Feng" uniqKey="Guo F" first="Feng" last="Guo">Feng Guo</name><name sortKey="Hao, Zhiyong" sort="Hao, Zhiyong" uniqKey="Hao Z" first="Zhiyong" last="Hao">Zhiyong Hao</name><name sortKey="He, Wei" sort="He, Wei" uniqKey="He W" first="Wei" last="He">Wei He</name><name sortKey="Jiang, Chengyu" sort="Jiang, Chengyu" uniqKey="Jiang C" first="Chengyu" last="Jiang">Chengyu Jiang</name><name sortKey="Kao, Richard Y" sort="Kao, Richard Y" uniqKey="Kao R" first="Richard Y" last="Kao">Richard Y. Kao</name><name sortKey="Kou, Binbin" sort="Kou, Binbin" uniqKey="Kou B" first="Binbin" last="Kou">Binbin Kou</name><name sortKey="Li, Li" sort="Li, Li" uniqKey="Li L" first="Li" last="Li">Li Li</name><name sortKey="Li, Qian" sort="Li, Qian" uniqKey="Li Q" first="Qian" last="Li">Qian Li</name><name sortKey="Liu, Gang" sort="Liu, Gang" uniqKey="Liu G" first="Gang" last="Liu">Gang Liu</name><name sortKey="Ni, Anping" sort="Ni, Anping" uniqKey="Ni A" first="Anping" last="Ni">Anping Ni</name><name sortKey="Rao, Shuan" sort="Rao, Shuan" uniqKey="Rao S" first="Shuan" last="Rao">Shuan Rao</name><name sortKey="Sun, Manji" sort="Sun, Manji" uniqKey="Sun M" first="Manji" last="Sun">Manji Sun</name><name sortKey="Tsui, Wayne H" sort="Tsui, Wayne H" uniqKey="Tsui W" first="Wayne H" last="Tsui">Wayne H. Tsui</name><name sortKey="Wang, Zhanguo" sort="Wang, Zhanguo" uniqKey="Wang Z" first="Zhanguo" last="Wang">Zhanguo Wang</name><name sortKey="Zhang, Huiyuan" sort="Zhang, Huiyuan" uniqKey="Zhang H" first="Huiyuan" last="Zhang">Huiyuan Zhang</name><name sortKey="Zhang, Liang" sort="Zhang, Liang" uniqKey="Zhang L" first="Liang" last="Zhang">Liang Zhang</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Hu, Hongbo" sort="Hu, Hongbo" uniqKey="Hu H" first="Hongbo" last="Hu">Hongbo Hu</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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